The goal of this project was to use ergonomic design principles to evaluate a 3-dimensional computer simulation of a manikin in a clinical laboratory setting for individuals with lower limb disabilities. The first step was to use the University of Michigan 3D Static Strength Prediction Program (3D SSPP) to determine biomechanical challenges from three simulated work postures commonly performed among clinical workers. Findings were then imported into the Purdue Envision Center’s 3D simulation of the Accessible Biomedical Immersion Laboratory (ABIL) located at Purdue’s Discovery and Learning Research Center (DLRC). Positions assumed in the 3D ABIL simulation for each task were predicted, using the 3D SSPP software, to be achievable by at least 90 percent of the population. Collection of data from four subjects performing standardized clinical tasks while in wheelchairs allowed for real-world validation of the 3D simulation model. Additional ergonomic analysis was conducted using the Rapid Upper Limb Assessment (RULA) tool along with subject questionnaires to determine upper limb work risk factors associated with clinical tasks and workstations. RULA and questionnaire data showed the presence of potential upper limb risk factors that may need to be incorporated into the 3D simulation model, especially during pipetting tasks when performed by female subjects. This pilot study showed promise as a clinical facility design tool. Further research is needed to fine tune the 3D ABIL simulation as a useful tool to create clinical facilities for those with lower-limb disabilities