The paper is dedicated to the problem of true and pseudoreplication of a biological experiment, in particular in the educational process. It was found that this issue is relatively new and actual for the methodology of biological experiments in general. Its solution in science ensures the veracity of the results obtained and the relevancy of the formulated conclusions. In national biology teaching methods at school, the problem of true and pseudoreplication of the experiment was not reflected. The author covers an issue of true replication teaching when setting up a model experiment to study genetic-evolutionary processes in populations. The paper discloses the experience in evolution of a model experiment and its development aimed at formation of ideas about technical and biological replication by the example of study of the genetic structure of an ideal population in generations. For this purpose, there was developed a web page that allows to automatically implement technical and biological experiment replication. There was described an experience of approbation of the proposed variant of the experiment, and its difficulties and advantages were revealed.