The paper aims to analyze the potential effects of truck platooning in terms of fuel consumption and CO2 emissions based on real demand data from the Italian highway system. The approach followed considers only vehicles that share the same entry and exit toll booths as platoonable, an assumption that, while ensuring manageable computational complexity, appears rather limiting for level 1 truck platooning. However, this approach seems highly plausible for level 2 and offers interesting policy insights.